Skin Cancer Charts Graphs: 11/09/08

Sunday, November 9, 2008

Fwd: Nuclear factor kappa B: A marker of chemotherapy for human stage IV gastric carcinoma.

---------- Forwarded message ----------
From: HubMed - cancer <rssfwd@rssfwd.com>
Date: Thu, Aug 28, 2008 at 6:28 AM
Subject: Nuclear factor kappa B: A marker of chemotherapy for human stage IV gastric carcinoma.
To: mesothelioma77@gmail.com

[1]World J Gastroenterol. 2008 Aug 14; 14(30): 4739-44
Long YM, Ye S, Rong J, Xie WR

AIM: To detect the nuclear factor kappa B (NF-kappaB) condition in human stage IV gastric carcinoma patients and to explore the correlation between NF-kappaB activation and survival of these patients after chemotherapy. METHODS: Expression of NF-kappaB-p65 was determined by immunohistochemical analysis. Activity of NF-kappaB DNA-binding in carcinoma tissue was detected by electrophoretic mobility shift assay. Kaplan-Meier survival analysis was performed to show the relation between NF-kappaB and progression-free survival (PFS) or overall survival (OS) of the patients. RESULTS: The positive expression rate of NF-kappaB-p65 in 60 gastric cancer tissue samples was 76.7% (46/60). The expression of NF-kappaB-p65 was reduced in adjacent carcinoma and normal tissue samples. Electrophoretic mobility shift assay (EMSA) analysis showed a strong activation of NF-kappaB in cancer tissue samples. A survival difference was found in NF-kappaB-p65 positive and negative patients. NF-kappaB-p65 expression was negative in cancer tissue samples (n = 14). PFS was 191.40 +/- 59.88 d and 152.93 +/- 16.99 d, respectively, in patients with positive NF-kappaB-p65 expression (n = 46) (P = 0.4028). The survival time of patients with negative and positive NF-kappaB-p65 expression was 425.16 +/- 61.61 d and 418.85 +/- 42.98 d, respectively (P = 0.7303). Kaplan-Meier analysis showed no significant difference in PFS or OS. The 46 patient tissue which positive NF-kappaB-p65 expression was found in the tissue samples from the 46 patients whose PFS and OS were 564.89 +/- 75.94 d and s 352.37 +/- 41.32 d, respectively (P = 0.0165). CONCLUSION: NF-kappaB is activated in gastric carcinoma tissue, which is related to the OS after chemotherapy.

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Source: http://www.hubmed.org/display.cgi?uids=18720533
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Fwd: OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models.

---------- Forwarded message ----------
From: HubMed - cancer <rssfwd@rssfwd.com>
Date: Thu, Aug 28, 2008 at 6:28 AM
Subject: OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models.
To: mesothelioma77@gmail.com

[1]World J Gastroenterol. 2008 Aug 14; 14(30): 4800-4
Shi J, Wei PK, Zhang S, Qin ZF, Li J, Sun DZ, Xiao Y, Yu ZH, Lin HM, Zheng GJ, Su XM, Chen YL, Liu YF, Xu L

AIM: To establish nude mouse human gastric cancer orthotopic transplantation models using OB glue paste technique. METHODS: Using OB glue paste technique, orthtopic transplantation models were established by implanting SGC-7901 and MKN-45 human gastric cancer cell strains into the gastric wall of nude mice. Biological features, growth of the implanted tumors, the success rate of transplantation and the rate of auto-metastasis of the two models were observed. RESULTS: The success rates of orthotopic transplan-tation of the two models were 94.20% and 96%. The rates of hepatic metastasis, pulmonary metastasis, peritoneal metastasis, lymphocytic metastasis and splenic metastasis were 42.13% and 94.20%, 48.43% and 57.97%, 30.83% and 36.96%, 67.30% and 84.06%, and 59.75% and 10.53%, respectively. The occurrence of ascites was 47.80% and 36.96%. CONCLUSION: OB glue paste technique is easy to follow. The biological behaviors of the nude mouse human gastric cancer orthotopic transplantation models established with this technique are similar to the natural processes of growth and metastasis of human gastric cancer, and, therefore, can be used as an ideal model for experimental research of proliferative metastasis of tumors.

___
Source: http://www.hubmed.org/display.cgi?uids=18720543
--

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Fwd: Gastric cancer cell lines induced by trichostatin A.

---------- Forwarded message ----------
From: HubMed - cancer <rssfwd@rssfwd.com>
Date: Thu, Aug 28, 2008 at 6:28 AM
Subject: Gastric cancer cell lines induced by trichostatin A.
To: mesothelioma77@gmail.com

[1]World J Gastroenterol. 2008 Aug 14; 14(30): 4810-5
Zou XM, Li YL, Wang H, Cui W, Li XL, Fu SB, Jiang HC

AIM: To explore the effect of trichostatin A (TSA) on apoptosis and acetylated histone H3 levels in gastric cancer cell lines BGC-823 and SGC-7901. METHODS: The effect of TSA on growth inhibition and apoptosis was examined by MTT, fluorescence microscopy and PI single-labeled flow cytometry. The acetylated histone H3 level was detected by Western blot. RESULTS: TSA induced apoptosis in gastric cancer cell lines BGC-823 and SGC-7901 was in a dose and time-dependent manner. Apoptotic cells varied significantly between TSA treated groups (37.5 ng/mL 72 h for BGC-823 cell line and 75 ng/mL 72 h for SGC-7901 cell line) and control group (0.85 +/- 0.14 vs 1.14 +/- 0.07, P = 0.02; 0.94 +/- 0.07 vs 1.15 +/- 0.06, P = 0.02). Morphologic changes of apoptosis, including nuclear chromatin condensation and fluorescence strength, were observed under fluorescence microscopy. TSA treatment in BGC-823 and SGC-7901 cell lines obviously induced cell apoptosis, which was demonstrated by the increased percentage of sub-G1 phase cells, the reduction of G1-phase cells and the increase of apoptosis rates in flow cytometric analysis. The result of Western blot showed that the expression of acetylated histone H3 increased in BGC-823 and SGC-7901 TSA treatment groups as compared with the control group. CONCLUSION: TSA can induce cell apoptosis in BGC-823 and SGC-7901 cell lines. The expression of acetylated histone H3 might be correlated with apoptosis.

___
Source: http://www.hubmed.org/display.cgi?uids=18720545
--

Enjoy Life. Think Today and Live Today!

Finance Issues Like; Life Insurance, Mortgage Refinancing, Auto Insurance, Student Loans, and Your Humor and Funny Stuffs and Games To Play.