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From: HubMed - cancer <rssfwd@rssfwd.com>
Date: Sat, Feb 16, 2008 at 8:08 PM
Subject: Analysis of p53 mutation status in human cancer cell lines: a paradigm for cell line cross-contamination.
To: mesothelioma77@gmail.com
[1]Cell Cycle. 2008 Feb 11; 7(5):
Berglind H, Pawitan Y, Kato S, Ishioka C, Soussi T
Cancer cell lines are essential tools used in many areas of biomedical research. Using the last release of the UMD_p53 database (2007)(1), we analysed the p53 status of 1,211 cell lines published between 1989 and 2007. p53 mutations in cell lines from various types of cancers display a striking similarity in the distribution of mutations and in the pattern of mutational events compared to tumours, indicating that they are representative of the cells from which they were derived. Analysis of the residual transcriptional activity of p53 mutants identified in cell lines that displayed two different p53 mutations show that there is a high frequency of weak mutations that are paired with more potent mutations suggesting a driver / passenger configuration. Strikingly, we found discrepancies in the p53 status for 23% (88/384) of cell lines, for which the p53 status was established.
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Source: http://www.hubmed.org/display.cgi?uids=18277095
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From: HubMed - cancer <rssfwd@rssfwd.com>
Date: Sat, Feb 16, 2008 at 8:08 PM
Subject: Analysis of p53 mutation status in human cancer cell lines: a paradigm for cell line cross-contamination.
To: mesothelioma77@gmail.com
[1]Cell Cycle. 2008 Feb 11; 7(5):
Berglind H, Pawitan Y, Kato S, Ishioka C, Soussi T
Cancer cell lines are essential tools used in many areas of biomedical research. Using the last release of the UMD_p53 database (2007)(1), we analysed the p53 status of 1,211 cell lines published between 1989 and 2007. p53 mutations in cell lines from various types of cancers display a striking similarity in the distribution of mutations and in the pattern of mutational events compared to tumours, indicating that they are representative of the cells from which they were derived. Analysis of the residual transcriptional activity of p53 mutants identified in cell lines that displayed two different p53 mutations show that there is a high frequency of weak mutations that are paired with more potent mutations suggesting a driver / passenger configuration. Strikingly, we found discrepancies in the p53 status for 23% (88/384) of cell lines, for which the p53 status was established.
___
Source: http://www.hubmed.org/display.cgi?uids=18277095
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Powered by [5]RssFwd, a service of [6]Blue Sky Factory, Inc