Fwd: Quantitative correlation between promoter methylation and messenger RNA levels of the reduced folate carrier.

---------- Forwarded message ----------
From: HubMed - breast cancer <rssfwd@rssfwd.com>
Date: Sat, May 3, 2008 at 11:09 PM
Subject: Quantitative correlation between promoter methylation and messenger RNA levels of the reduced folate carrier.
To: mesothelioma77@gmail.com


[1]BMC Cancer. 2008 May 1; 8(1): 124
Yang R, Li WW, Hoang BH, Kim H, Banerjee D, Kheradpour A, Healey JH, Meyers PA, Bertino JR, Gorlick R

ABSTRACT: BACKGROUND: Methotrexate (MTX) uptake is mediated by the reduced folate carrier (RFC). Defective drug uptake in association with decreased RFC expression is a common mechanism of MTX resistance in many tumor types. Heavy promoter methylation was previously identified as a basis for the complete silencing of RFC in MDA-MB-231 breast cancer cells, its role and prevalence in RFC transcription regulation are, however, not widely studied. METHODS: In the current study, RFC promoter methylation was assessed using methylation specific PCR in a panel of malignant cell lines (n = 8), including MDA-MB-231, and M805, a MTX resistant cell line directly established from the specimen of a patient with malignant fibrohistocytoma, whom received multiple doses of MTX. A quantitative approach of real-time PCR for measuring the extent of RFC promoter methylation was developed, and was validated by direct bisulfite genomic sequencing. RFC mRNA levels were determined by quantitative real-time RT-PCR and were related to the extent of promoter methylation in these cell lines. RESULTS: A partial promoter methylation and RFC mRNA down-regulation were observed in M805. Using the quantitative approach, a reverse correlation (correlation coefficient = -0.59, p

___
Source: http://www.hubmed.org/display.cgi?uids=18452618
--
 Powered by [5]RssFwd, a service of [6]Blue Sky Factory, Inc

 

Your Finance:

Sitemap

Debts, Loans And Consolidation Tips, Guide, Strategies And Info:

Debts and Loan Consolidation

Managing Your Debts

Debt Consolidation Loan

Consolidate Debt Loans: For Better Debt Management

Consolidate Debt Loans: To Eliminate Credit Card Debts

Consolidate Debt Loans

Consolidate Debt Loans For Your School Debts

Debt Consolidation UK

Debt Consolidation UK; Secured and Unsecured Debt Consolidation Explained

Private Student Loans: A Parent's Best Choice?

 College Consolidation Loans

School Consolidation Loan Guide

 School Loan Consolidation

School Loan Consolidation: Your Much Needed Information

 School Consolidation Loan

 Enter Bankruptcy Protection

Bankruptcy Mailing List

 Bankruptcy Marketing

Your Mortgage Guide And Info

Mortgage Loans

Mortgage Refinancing

How Much Mortgage Can I Afford?

Best Mortgage Rates Refinancing

Low Mortgage Rate Refinance

Connecticut Mortgage Refinancing

Mortgage Loan Refinancing In Britain

UK Mortgage Payment Calculator

Arkansas Refinance Mortgage Rates

Home Loan Rates Berkeley Springs

Home Loan Rates WV

Mortgage Rate History

What is Subprime Mortgage?

Foreclosures Tips, Guide, Strategies and Info Sites

Foreclosures

Foreclosures Bank Owned

Mortgage Foreclosure Appraisal

Property Foreclosures

Reasons Foreclosures Occur

Deed In Lieu Of Foreclosure

Regulations Preventing Foreclosure

Your Real Estate Tips, Guide, Strategies And Info:

Real Estate: Important Things to Remember

MS Beach Vacation Condos

Real Estate Marbella

Franklin TN Real Estate

Hud Homes Savannah Georgia

Margate Real Estate

Hud Approved Builders

It's All About Your Insurance

Insurance Premiums and Your DUI, DWI

The Role of Insurance

Insurance Defined

Low Car Insurance Rate Canada

Your DWI And DUI Tips, Guide And Info:

97402 Dui Classses

Austin DWI Attorney Info

Florida DWI Arrest

Austin DWI and DUI Defined

Florida Dwi Defense

Your Pet Dogs Needs, Tips, Guide, Strategies, and Info

Pet Dogs Wellness

Air Filled Dog Bed

Transport Dog Crates

Dog Transport

Air Filled Dog Bed: Your Best Friends Comfort

 Unique Dog Collars

Dog Crate Training

Popular Transport Dog Crates

Puppy Colic

Pet Dogs: Breeds With Behavioral Problems?

Your Health Tips, Guide and Info

Health Wellness

Relieve Stress Anxiety Tensions

Holiday Stress

Mind Body Yoga

Mesothelioma

Mesothelioma diagnosis: What You Need To Know

Daily Recommended Relaxation

Transcendental Meditation

More Articles For You

Related Articles

http://www.lingwellness.com/foreclosures.php

http://www.lingwellness.com/reasonsforeclosuresoccur.php

http://www.lingwellness.com/ukmortgagepaymentcalculator.php

http://www.lingwellness.com/homeloanrateswv.php

http://www.lingwellness.com/mortgageratehistory.php

http://www.lingwellness.com/lowmortgageraterefinance.php

http://www.lingwellness.com/connecticutmortgagerefinancing.php

http://www.lingwellness.com/arkansasrefinancemortgagerates.php

http://www.lingwellness.com/homeloanratesberkeleysprings.php

http://www.lingwellness.com/bestmortgageratesrefinancing.php

http://www.lingwellness.com/howmuchmortgagecaniafford.php

http://www.lingwellness.com/mortgagerefinancing.php

http://www.lingwellness.com/realestate.php

http://www.lingwellness.com/msbeachvacationcondos.php

http://www.lingwellness.com/margaterealestate.php

http://www.lingwellness.com/hudapprovedbuilders.php

http://www.lingwellness.com/bankruptcymarketing.php

http://www.lingwellness.com/bankruptcymailinglist.php

http://www.lingwellness.com/enterbankruptcyprotection.php

http://www.lingwellness.com/debtconsolidationuk2.php

http://www.debt2consolidationloan.com/

http://www.debt2consolidationloan.com/Consolidate_Debt_Loans_The_Right_Type_of_Debt_Consolidation_Loan.html

http://www.debt2consolidationloan.com/

http://www.debt2consolidationloan.com/School_Loan_Consolidation_Beginners_Info.html

http://www.debt2consolidationloan.com/School_Consolidation_Loans_Guide.html

http://www.debt2consolidationloan.com/College_Loan_Consolidation.html

http://www.debt2consolidationloan.com/Student_Loans.html

http://www.lingwellness.com

http://www.lingwellness.com/howmuchmortgagecaniafford.php

http://www.lingwellness.com/floridadwidefense.php

http://www.lingwellness.com/bestmortgageratesrefinancing.php

 http://www.lingwellness.com/arkansasrefinancemortgagerates.php

http://www.lingwellness.com/howmuchmortgagecaniafford.php

http://www.lingwellness.com/petdogs.php

http://www.lingwellness.com/transportdogcrates.php

http://www.lingwellness.com/airfilleddogbed.php

http://www.lingwellness.com/dogtransport.php

http://www.lingwellness.com/uniquedogcollars.php

http://www.lingwellness.com/airfilleddogbedyourbestfriendscomfort.php

http://www.lingwellness.com/dogcratetraining.php

http://www.lingwellness.com/populartransportdogcrates.php

http://www.lingwellness.com/puppycolic.php