---------- Forwarded message ----------
From: HubMed - cancer <rssfwd@rssfwd.com>
Date: Sun, Jun 15, 2008 at 11:30 AM
Subject: Bisulfite modification for analysis of DNA methylation.
To: mesothelioma77@gmail.com
[1]Curr Protoc Nucleic Acid Chem. 2008 Jun; Chapter 6: Unit 6.10
Hayatsu H, Shiraishi M, Negishi K
Bisulfite is known to deaminate cytosine in nucleic acids, while 5-methylcytosine resists this bisulfite action. For this reason, bisulfite treatment has been used for detecting 5-methylcytosine in DNA, a minor component of eukaryotic DNA, presently recognized as playing an important role in the control of gene function. This procedure, called bisulfite genomic sequencing, is a principal method for the analysis of DNA methylation in various biological phenomena, including human diseases such as cancer. This unit describes an efficient procedure utilizing a newly developed high-concentration bisulfite solution. Protocols for this methodology are supplemented with discussions focused on chemical aspects of the bisulfite treatment.
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Source: http://www.hubmed.org/display.cgi?uids=18551428
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From: HubMed - cancer <rssfwd@rssfwd.com>
Date: Sun, Jun 15, 2008 at 11:30 AM
Subject: Bisulfite modification for analysis of DNA methylation.
To: mesothelioma77@gmail.com
[1]Curr Protoc Nucleic Acid Chem. 2008 Jun; Chapter 6: Unit 6.10
Hayatsu H, Shiraishi M, Negishi K
Bisulfite is known to deaminate cytosine in nucleic acids, while 5-methylcytosine resists this bisulfite action. For this reason, bisulfite treatment has been used for detecting 5-methylcytosine in DNA, a minor component of eukaryotic DNA, presently recognized as playing an important role in the control of gene function. This procedure, called bisulfite genomic sequencing, is a principal method for the analysis of DNA methylation in various biological phenomena, including human diseases such as cancer. This unit describes an efficient procedure utilizing a newly developed high-concentration bisulfite solution. Protocols for this methodology are supplemented with discussions focused on chemical aspects of the bisulfite treatment.
___
Source: http://www.hubmed.org/display.cgi?uids=18551428
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Powered by [5]RssFwd, a service of [6]Blue Sky Factory, Inc