Skin Cancer Charts Graphs: Fwd: Isolation of RNA from residual BD SurePathtrade mark liquid-based cytology specimens and detection of HPV E6/E7 mRNA using the PreTecttrade mark HPV-Proofer assay.

Sunday, September 14, 2008

Fwd: Isolation of RNA from residual BD SurePathtrade mark liquid-based cytology specimens and detection of HPV E6/E7 mRNA using the PreTecttrade mark HPV-Proofer assay.

---------- Forwarded message ----------
From: HubMed - cancer <rssfwd@rssfwd.com>
Date: Tue, Sep 2, 2008 at 8:26 PM
Subject: Isolation of RNA from residual BD SurePathtrade mark liquid-based cytology specimens and detection of HPV E6/E7 mRNA using the PreTecttrade mark HPV-Proofer assay.
To: mesothelioma77@gmail.com

[1]J Virol Methods. 2008 Aug 28;
Dixon EP, King LM, Adams MD, Grønn P, Murphy PG, Brown CA, Brough GH, Skomedal H, Malinowski DP, Fischer TJ

Infection with high-risk human papillomavirus (HPV) is known to be associated directly with the development of cervical cancer. Recent data suggests that the detection of E6/E7 mRNA from high-risk HPV types may serve as a better diagnostic method for detecting the presence of cervical pre-cancer than HPV DNA testing. This report details a commercially available nucleic acid isolation protocol which can be used to isolate reproducibly RNA from residual BD SurePathtrade mark liquid-based cytology specimens stored for up to 28 days, and have demonstrated the quality and quantity of mRNA is sufficient for detection with the NorChip PreTecttrade mark HPV-Proofer assay. Of the 242 specimens tested in this study, 236 (97.5%) tested positive for U1A internal control gene expression. HPV type 16, 18, 31, 33 or 45 mRNA was detected in 16/20 (80%) of the analyzed high-grade squamous intraepithelial lesion (HSIL) specimens, with a low frequency of HPV mRNA detected in the normal lesions (3%). The presence of HPV E6 expression in a subset of HPV positive specimens was also detected by real-time RT-PCR. These findings confirm that RNA of sufficient quality can be isolated from residual BD SurePathtrade mark cervical cytology specimens for use in downstream NASBA and RT-PCR-based assays.

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Source: http://www.hubmed.org/display.cgi?uids=18761379
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