Skin Cancer Charts Graphs: Fwd: Denaturational Stress Induces Formation of Zinc-Deficient Monomers of Cu,Zn Superoxide Dismutase: Implications for Pathogenesis in Amyotrophic Lateral Sclerosis.

Friday, September 12, 2008

Fwd: Denaturational Stress Induces Formation of Zinc-Deficient Monomers of Cu,Zn Superoxide Dismutase: Implications for Pathogenesis in Amyotrophic Lateral Sclerosis.

---------- Forwarded message ----------
From: HubMed - cancer <rssfwd@rssfwd.com>
Date: Tue, Sep 2, 2008 at 8:26 PM
Subject: Denaturational Stress Induces Formation of Zinc-Deficient Monomers of Cu,Zn Superoxide Dismutase: Implications for Pathogenesis in Amyotrophic Lateral Sclerosis.
To: mesothelioma77@gmail.com

[1]J Mol Biol. 2008 Aug 22;
Mulligan VK, Kerman A, Ho S, Chakrabartty A

Mutations in the Cu,Zn superoxide dismutase (SOD1) cause a subset of amyotrophic lateral sclerosis cases. SOD1 is a homodimer in which each monomer binds one copper atom and one zinc atom. Mutation is believed to increase the conformational flexibility of SOD1, giving rise to a misfolded SOD1 population with novel cytotoxic properties. While SOD1's metal ligands affect its stability greatly, little is known about the role these metals play in the folding, unfolding, and misfolding processes. Here, we present a method by which we were able to measure the rates of metal release during SOD1 unfolding in guanidine hydrochloride. Rates of dimer dissociation, measured by a time-resolved cross-linking assay, and conformational changes in SOD1's beta-barrel core, monitored by tryptophan fluorescence intensity, were compared with the rates of copper release and zinc release. Correlations were observed across a range of denaturant concentrations, giving rise to a more detailed model of the SOD1 unfolding process than was previously available. According to this model, the major unfolding pathway involves simultaneous dimer dissociation and zinc release as an early step that is followed by a slow conformational change in the protein's core, which, in turn, is followed by rapid copper release. This model establishes a zinc-deficient, copper-loaded SOD1 monomer as a well-populated SOD1 unfolding intermediate and a species likely to be populated under conditions of denaturational stress. Because the cytotoxicity of zinc-deficient SOD1 has been demonstrated previously, this species is a good candidate for the cytotoxic species in SOD1-associated amyotrophic lateral sclerosis.

___
Source: http://www.hubmed.org/display.cgi?uids=18761352
--
Powered by [5]RssFwd, a service of [6]Blue Sky Factory, Inc

Freshen Your Day. Watch and Upload Some Of Your Humor And Funny Stuffs, Funny Videos

More Links and Info Sites For Your Searches

Search Info Source

Humor and Funny Stuffs

Funny Tagalog Quotes, Funny Short Skits, Funny Car Commercials

I Need Some Funny Jokes Riddles For Teenagers, Really Funny Quick Jokes

Funny Goodbye Letters, Funny Rude Free Ecards, Funny Sick Twisted Humor

Funny Videos and Entertainment

Funny Videos

What Is The Average Price of Life Insurance

How Much Does Life Insurance Cost